These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Establishment of highly enriched type-2 astrocyte cultures and quantitative determination of intense glutamine synthetase activity in these cells.
    Author: Juurlink BH, Hertz L.
    Journal: J Neurosci Res; 1991 Nov; 30(3):531-9. PubMed ID: 1686919.
    Abstract:
    We report procedures that allow one to develop and maintain cultures highly enriched in rat neopallial type-2 astrocytes. Even after four weeks such cultures consist of more than 90% type-2 astrocytes, approximately 5% O-2A progenitors and fewer than 2% type-1 astrocytes. Their survival for more than 5 days requires the addition of conditioned medium from type-1 astrocyte cultures. The type-2 astrocytes have an intense glutamine synthetase activity whose basal level is sevenfold higher than in type-1 astrocytes. The glutamine synthetase activities of both the type-2 and type-1 astrocytes are increased after exposure to cortisol. Thus, type-2 astrocytes express the two quint-essential astrocytic features: glial fibrillary acidic protein (previously reported by others) and glutamine synthetase.
    [Abstract] [Full Text] [Related] [New Search]