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  • Title: Diagnosis of human rotavirus in stool specimens: comparison of different methods.
    Author: Paul SK, Tabassum S, Islam MN, Ahmed MU, Haq JU, Shamsuzzaman AK.
    Journal: Mymensingh Med J; 2006 Jul; 15(2):183-7. PubMed ID: 16878102.
    Abstract:
    A total of 345 stool specimens of hospitalized children below 5 years of age with acute gastroenteritis were tested for the presence of rotavirus by Polyacrylamide gel electrophoresis (PAGE), a monoclonal antibody based enzyme immunoassay (EIA) and a latex agglutination test (LAT). Detection rate for PAGE and EIA were 24.9% (345/86) and 20.9% (345/70) respectively. Using PAGE as the standard, the sensitivity and specificity of EIA were 75.6% and 98.1% respectively. The sensitivity of LAT was 70.9% with 100% specificity (LAT was done in only PAGE positive specimens). LAT appeared as the simplest and economic for both bed side and field use.
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