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  • Title: Direct molecular approach to monitoring bacterial colonization on vacuum-packaged beef.
    Author: Fontana C, Cocconcelli PS, Vignolo G.
    Journal: Appl Environ Microbiol; 2006 Aug; 72(8):5618-22. PubMed ID: 16885317.
    Abstract:
    Denaturing gradient gel electrophoresis allowed us to monitor total bacterial communities and to establish a pattern of succession between species in vacuum-packaged beef stored at 2 and 8 degrees C for 9 weeks and 14 days. Species-specific PCR was used to confirm the presence of Lactobacillus sakei and Lactobacillus curvatus. Multiplex PCRs using 16S rRNA-specific primers allowed differentiation between Leuconostoc species. These methods provided the desired information about microbial diversity by detecting the main microorganisms capable of colonizing this ecological niche.
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