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  • Title: Characterization of the Drosophila melanogaster ribosomal proteome.
    Author: Alonso J, Santarén JF.
    Journal: J Proteome Res; 2006 Aug; 5(8):2025-32. PubMed ID: 16889426.
    Abstract:
    We have combined high-resolution two-dimensional (2-D) gel electrophoresis with mass spectrometry to identifying proteins represented in a 2-D gel database of Drosophila melanogaster ribosomes. First, we purified ribosomes from third instar Drosophila larvae and constructed a high-resolution 2-D gel database containing 58 Coomassie blue stained polypeptides. Next, we carried out preparative 2-D PAGE to isolate some of the polypeptides and characterize them by MALDI-TOF. Using this strategy we identified 52 ribosomal spots in the database, and in each case confirmed their identity by MALDI-TOF/TOF. The database can be used to analyze Minute mutants of Drosophila.
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