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  • Title: Fast and precise protein tracking using repeated reversible photoactivation.
    Author: Chudakov DM, Chepurnykh TV, Belousov VV, Lukyanov S, Lukyanov KA.
    Journal: Traffic; 2006 Oct; 7(10):1304-10. PubMed ID: 16889652.
    Abstract:
    Photoactivatable fluorescent proteins opened principally novel possibilities to study proteins' movement pathways. In particular, reversibly photoactivatable proteins enable multiple tracking experiments in a long-drawn work with a single cell. Here we report 'protein rivers tracking' technique based on repeated identical rounds of photoactivation and subsequent images averaging, which results in dramatic increase of imaging resolution for fast protein movement events.
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