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  • Title: [The estimation of Pneumocystis carinii in HIV-infected patients occurrence based on three different methods].
    Author: Ciarkowska J, Spausta G, Wiczkowski A, Adamek B, Beniowski M.
    Journal: Wiad Parazytol; 2001; 47(3):543-8. PubMed ID: 16894772.
    Abstract:
    The frequency of Pneumocystis carinii occurrence in BAL of 38 HIV-infected patients was determined with three different method. BAL sediments were stained with Giemsa method, silvered according to Gomori-Grocott method and studied with indirect immunofluorescence assay. Using Giemsa method staining Pneumocystis carinii was diagnosed in 81.6% of patients, in Gomori-Grocott method--in 31.6% of patients, but results of indirect immunofluorescence assay were positive only in 23.,7%. In our study staining BAL sediments with Giemsa method allowed to detect Pneumocystis carinii in the highest percentage of examined patients.
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