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  • Title: [Porcine follistatin cDNA cloning and expression in Escherichia coli].
    Author: He X, Qi B, He LQ, Chen YF, Liu GS, Chen QX.
    Journal: Sheng Wu Gong Cheng Xue Bao; 2006 Jul; 22(4):677-81. PubMed ID: 16894909.
    Abstract:
    The total RNA was extracted from porcine ovary. Porcine Follistatin cDNA was cloned by RT-PCR. Complete porcine follistatin cDNA coding sequences are presented including 1038 bp of open reading frame. The purified porcine follistatin cDNA was inserted into pGEX-4T-3 vector to construct the prokaryotic fusion protein expression vector. The recombinant expression plasmid was transformed into BL21 (DE3) and expression was induced by IPTG. Protein products were detected by SDS-PAGE and confirmed by Western blotting analysis, which showed that the yield of the Follistatin cDNA was a 63kD protein expression vector. Follistatin protein was expressed in the form of glutathione-S-transferase (GST) fusion protein in E. coli.
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