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  • Title: Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine-tagged proteins.
    Author: Lee IS, Lee N, Park J, Kim BH, Yi YW, Kim T, Kim TK, Lee IH, Paik SR, Hyeon T.
    Journal: J Am Chem Soc; 2006 Aug 23; 128(33):10658-9. PubMed ID: 16910642.
    Abstract:
    Ni/NiO core/shell nanoparticles having high affinity with polyhistidine were synthesized by decomposition of a Ni surfactant complex followed by air oxidation. Ni/NiO nanoparticles showed selective and efficient binding to histidine-tagged proteins and easy separation by using a magnet. These provided a more convenient way to efficient purification of histidine-tagged proteins compared with the conventional Ni-NTA complex-bound resins and microbeads.
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