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  • Title: Determination of proteins in infant formula by high-performance liquid chromatography-electrospray tandem mass spectrometry.
    Author: Hong SS, Park JH, Kwon SW.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2007 Jan 01; 845(1):69-73. PubMed ID: 16911878.
    Abstract:
    To determine the protein content of formula, gel electrophoresis was performed on the infant formula samples and the entire protein patterns were analyzed by nano-high performance liquid chromatography-electrospray tandem mass spectrometry (nano-HPLC/ESI/MS/MS). From the commercial infant formula profiled in this study, a total of 154 peptides, corresponding to 31 unique proteins were identified by nano-HPLC/ESI/MS/MS. Each of the identified peptides was reconfirmed by a strict integrated approach using tandem mass spectra. This protein profiling method using gel electrophoresis coupled with nano-HPLC/ESI/MS/MS and manual evaluation is a sensitive and accurate method for protein identification as well as a powerful tool for monitoring various types of food products.
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