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  • Title: The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease.
    Author: Levitz R, Chapman D, Amitsur M, Green R, Snyder L, Kaufmann G.
    Journal: EMBO J; 1990 May; 9(5):1383-9. PubMed ID: 1691706.
    Abstract:
    The optional Escherichia coli prr locus restricts phage T4 mutants lacking polynucleotide kinase or RNA ligase. Underlying this restriction is the specific manifestation of the T4-induced anticodon nuclease, an enzyme which triggers the cleavage-ligation of the host tRNALys. We report here the molecular cloning, nucleotide sequence and mutational analysis of prr-associated DNA. The results indicate that prr encodes a latent form of anticodon nuclease consisting of a core enzyme and cognate masking agents. They suggest that the T4-encoded factors of anticodon nuclease counteract the prr-encoded masking agents, thus activating the latent enzyme. The encoding of a tRNA cleavage-ligation pathway by two separate genetic systems which cohabitate E. coli may provide a clue to the evolution of RNA splicing mechanisms mediated by proteins.
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