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  • Title: [Detecting poliovirus in wastewater from Armenia, Colombia].
    Author: González MM, Sarmiento L, Castaño JC, Giraldo AM, Salazar A, Muñoz NJ.
    Journal: Rev Salud Publica (Bogota); 2006 May; 8 Suppl 1():13-23. PubMed ID: 16925118.
    Abstract:
    OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater. METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers. RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country. CONCLUSION: Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.
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