These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Cloning and characterization of porcine Fc gamma receptor II (FcgammaRII).
    Author: Qiao S, Zhang G, Xia C, Zhang H, Zhang Y, Xi J, Song H, Li X.
    Journal: Vet Immunol Immunopathol; 2006 Nov 15; 114(1-2):178-84. PubMed ID: 16930721.
    Abstract:
    Receptors for the Fc region (FcgammaRs) of IgG play a crucial role in the immune system and host protection against infection. In the present study, we describe the cloning, sequencing and characterization of porcine FcgammaRII. By screening a translated EST database with the protein sequence of the human FcgammaRII (CD32) we identified a putative porcine homologue. Using rapid amplification of cDNA ends (RACE), we isolated the full-length cDNA encoding porcine FcgammaRII from peripheral blood leucocyte RNA. The porcine FcgammaRII cDNA was 1488bp long, encoding a 297 amino acid trans-membrane glycoprotein composed of two immunoglobulin-like extracelluar domains, a trans-membrane region and a cytoplasmic tail with an immunoreceptor tyrosine-based inhibitory motif (ITIM). The predicted amino acid sequence was found to be 67% and 52% identitical with human and mouse FcgammaRIIB. RT-PCR indicated porcine FcgammaRII transcripts expressed in liver, alveolar, mesenteric lymph node and PBLs. COS-7 cells transfected with the pig FcgammaRII cDNA were able to bind chicken erythrocytes sensitized with porcine IgG. Identification of porcine FcgammaRII will aid in the understanding IgG-FcgammaR interactions, and may help in developing new immunization protocols.
    [Abstract] [Full Text] [Related] [New Search]