These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Chromatographic recovery of polypeptides from copper-stained sodium dodecyl sulfate polyacrylamide gels.
    Author: Vanfleteren JR, Peeters K.
    Journal: J Biochem Biophys Methods; 1990 Mar; 20(3):227-35. PubMed ID: 1693157.
    Abstract:
    A procedure of preparative electrophoresis is described in which proteins separated on sodium dodecyl sulfate gels, stained with copper and eluted by simple diffusion, are highly concentrated on a fluorocarbon packing and freed of small molecular weight substances, including sodium dodecyl sulfate and buffer components and gel-related substances. This method can be used for microscale preparations or it can be scaled up to recover milligram amounts of protein. The purified polypeptides, however denatured, are suitable for amino acid sequencing.
    [Abstract] [Full Text] [Related] [New Search]