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  • Title: Development and validation of a HPLC method for the quantitation of ochratoxins in plasma and raw milk.
    Author: Boudra H, Morgavi DP.
    Journal: J Chromatogr B Analyt Technol Biomed Life Sci; 2006 Nov 07; 843(2):295-301. PubMed ID: 16939716.
    Abstract:
    A HPLC method with improved sensitivity for the determination of ochratoxins (OT) A, B and alpha in plasma and milk was developed. Plasma analysis involved a simple liquid-liquid extraction with chloroform; while for milk, an additional immunoaffinity clean-up step was necessary. The method showed a good linearity (r(2)>0.999). The limit of quantification (LOQ) of OTA was 5 and 200 ng/l for milk and plasma, respectively. Average recovery was 89% in both matrices, except for OTalpha in milk that was only 18% due to poor immunoaffinity binding. OT remained stable in -20 degrees C stored samples; OTA concentration in plasma and milk did not change after 8 and 18 months of storage, respectively. The developed method has been applied to contaminated plasma and milk samples obtained from dairy ewes fed with ochratoxin-contaminated feed.
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