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  • Title: Refolding and purification of recombinant human VEGF-121 expressed as inclusion bodies in Escherichia coli.
    Author: Hu ZM, Ma L, Zhou MQ, Gao JM, Wang XN.
    Journal: Nan Fang Yi Ke Da Xue Xue Bao; 2006 Aug; 26(8):1083-6. PubMed ID: 16939889.
    Abstract:
    Vascular endothelial growth factor 121 (VEGF(121)) was expressed as inclusion bodies by recombinant Escherichia coli. High concentrations of both biomass (46 g dry cell/L) and VEGF(121) inclusion bodies (4.5 g/L) were obtained by applying a high-cell-density culture. After the inclusion bodies were washed and dissolved, VEGF(121) was refolded at 0.2 mg/ml by ultrafiltration in refolding buffer with a yield of 81%. Renatured VEGF(121) was purified by anion chromatography and Sephacry S-100 chromatography with purity higher than 95% and final purification yield of 31%. The purified VEGF(121) could stimulate the proliferation of human umbilical vein endothelial cells as demonstrated by a biological activity assay.
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