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  • Title: Comparison of MS/MS methods for protein identification from 2D-PAGE.
    Author: Arrigoni G, Fernandez C, Holm C, Scigelova M, James P.
    Journal: J Proteome Res; 2006 Sep; 5(9):2294-300. PubMed ID: 16944941.
    Abstract:
    We have compared the use of a low resolution MALDI-Ion Trap MS/MS and a high-resolution ESI-TOF-MS/MS for the analysis of spots from 2D gels. The main criteria were speed and accuracy of protein identification. The results obtained using the MALDI-MS/MS system are comparable to those from the LC-MS/MS system in terms of accuracy, but less low-level proteins are identified while the time required for the analysis is dramatically reduced.
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