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  • Title: The myelin-associated glycoprotein is phosphorylated in the peripheral nervous system.
    Author: Agrawal HC, Noronha AB, Agrawal D, Quarles RH.
    Journal: Biochem Biophys Res Commun; 1990 Jun 29; 169(3):953-8. PubMed ID: 1694664.
    Abstract:
    Phosphorylation of the myelin-associated glycoprotein (MAG) in the peripheral nervous system is demonstrated by immunoprecipitation from myelin proteins radiolabeled in vivo, in nerve slices and in a cell-free system. Phosphoamino acid analysis of immunoprecipitated MAG revealed the presence of radioactivity in phosphoserine, but not in phosphothreonine or phosphotyrosine. Only the shorter isoform of MAG (S-MAG) was detected by immunostaining of nitrocellulose sheets with anti-MAG anti-serum after enzymatic deglycosylation of immunoprecipitated MAG labeled in nerve slices. Autoradiography of the same Western blots revealed that most of the radioactive phosphate was in S-MAG, demonstrating that the polypeptide backbone of S-MAG is phosphorylated in the PNS.
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