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  • Title: Autologous heterotopic transplantation of intact rabbit ovary after frozen banking at -196 degrees C.
    Author: Chen CH, Chen SG, Wu GJ, Wang J, Yu CP, Liu JY.
    Journal: Fertil Steril; 2006 Oct; 86(4 Suppl):1059-66. PubMed ID: 16949589.
    Abstract:
    OBJECTIVE: To determine the efficiency of intact ovary after cryopreservation and transplantation in a rabbit model for limited longevity by revascularization ischemia after implantation of frozen-thawed ovarian tissue. Transplantation of an intact frozen ovary with microvascular anastomos has been shown to be feasible with modern cryobiology and microsurgery. DESIGN: Animal study. SETTING: University-based teaching hospital. ANIMAL(S): Twelve 5-month-old mature female New Zealand White rabbits. INTERVENTION(S): After bilateral oophorectomy with one ovary saved as a control in formalin-fixed and hematoxylin and eosin-stained paraffin sections, the contralateral intact ovary was used for autologous heterotopic transplantation after freezing and thawing. MAIN OUTCOME MEASURE(S): Density of primordial follicles 6 months after transplantation was measured. Hormone levels and vaginal cytology were followed throughout the oophorectomy and transplantation period. RESULT(S): Ten of 12 rabbits had restored ovarian function 1 week after transplantation of their intact cryopreserved ovary. At the 6-month posttransplantation follow-up, mean primordial follicle density was statistically significantly lower in the experimental than in the control ovaries (13.99 +/- 3.21 vs. 18.68 +/- 3.86 per high-power field). The remaining two rabbits, which had cracked mesentery fat adjacent to the ovary after thawing, never regained ovarian function. CONCLUSION(S): With microsurgery, cryopreservation of an intact rabbit ovary followed by autologous transplantation may overcome revascularization-associated ischemia to save primordial follicles and attain reasonable graft longevity.
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