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  • Title: T7 RNA polymerase: use of limited proteolysis for the study of enzyme's interaction with substrates and inhibitors.
    Author: Tunitskaya VL, Akbarov AKh, Kochetkov SN.
    Journal: Biochem Int; 1990; 20(6):1033-40. PubMed ID: 1695096.
    Abstract:
    The specific limited trypsinolysis of bacteriophage T7 RNA polymerase (T7RP) was performed in the presence of various components of the polymerase reaction and some GTP-analogs--irreversible inhibitors of the enzyme. The differences in the rate and sites of proteolysis were observed. Basing on the data obtained the role of the N-terminal domain of the T7RP in the interaction with promoter-containing template is proposed.
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