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  • Title: Crystallization and preliminary diffraction studies of recombinant human granulocyte-stimulating factor (KW2228).
    Author: Nagahara Y, Konishi N, Yokoo Y, Hirayama N.
    Journal: J Mol Biol; 1990 Jul 05; 214(1):25-6. PubMed ID: 1695250.
    Abstract:
    Human granulocyte colony-stimulating factor (hG-CSF) specifically stimulates proliferation of neutrophils. Two crystal forms of a mutant of hG-CSF expressed in Escherichia coli have been obtained using the hanging drop vapour diffusion method. One form is triclinic, space group P1, with cell dimensions a = 37.3 A, b = 46.4 A, c = 47.7 A, alpha = 105.5 degrees, beta = 98.0 degrees and gamma = 109.4 degrees. The other is monoclinic, space group C2, with cell dimensions a = 82.0 A, b = 49.2 A, c = 49.4 A and beta = 113.9 degrees. Both crystal forms diffract beyond 2.0 A and are suitable for X-ray analysis.
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