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  • Title: Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120.
    Author: Olmedo-Verd E, Muro-Pastor AM, Flores E, Herrero A.
    Journal: J Bacteriol; 2006 Sep; 188(18):6694-9. PubMed ID: 16952962.
    Abstract:
    The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N(2)-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P(2)) and promoters that are induced upon N step-down (P(1) and P(3)). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P(3) and P(1).
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