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  • Title: Induction of F9 cell differentiation by transient exposure to retinoic acid.
    Author: Dong JM, Li F, Chiu JF.
    Journal: Biochem Biophys Res Commun; 1990 Jul 16; 170(1):147-52. PubMed ID: 1695505.
    Abstract:
    The F9 cell is a mouse embryonal teratocarcinoma which can be induced to differentiate into visceral endoderm by treatment with retinoic acid (RA). Treatment with RA in conventional studies was carried out in the constant presence of RA. Here we demonstrate that treatment with RA can be as short as 3 hrs to induce differentiation of F9 cells. Morphology, alpha-fetoprotein gene activity, and temporal patterns of F9 cell differentiation are the same with both short- and long-term treatment with RA.
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