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  • Title: Identification and differentiation of Legionella pneumophila and Legionella spp. with real-time PCR targeting the 16S rRNA gene and species identification by mip sequencing.
    Author: Stølhaug A, Bergh K.
    Journal: Appl Environ Microbiol; 2006 Sep; 72(9):6394-8. PubMed ID: 16957269.
    Abstract:
    Fluorescent resonance energy transfer probes targeting the 16S rRNA gene were constructed for a sensitive and specific real-time PCR for identification and differentiation of Legionella pneumophila from other Legionella spp. For identification of non-L. pneumophila spp. by direct amplicon sequencing, two conventional PCR assays targeting the mip gene were established.
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