These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Fetal D gene in maternal plasma should be examined in all immunized Rh(-) pregnant women to avoid invasive procedures].
    Author: Zupańska B, Orzińska A, Nowaczek-Migas M, Guz K, Wielgoś M, Debski R, Gibała A, Rumas-Bec J, Brojer E.
    Journal: Ginekol Pol; 2006 May; 77(5):359-64. PubMed ID: 16958225.
    Abstract:
    UNLABELLED: We have recently developed and published a noninvasive determination of fetal RhD status by examination of cell-free DNA in maternal plasma. The predictive value of the procedure of fetal testing, already published by us, was 99,6%. AIM: To assess the necessity of RhD fetal testing in immunized Rh(-) mothers with Rh(+) partners. MATERIAL: Rh(-) mothers with anti-D antibodies, their partners and children. METHODS: Molecular: RHD gene examination by real-time polymerase chain reaction; analysis of control genes present in the father but not in the mother. Serological: titre of anti-D antibodies, Rh phenotypes. RESULTS: Among 53 Rh(+) partners of immunized Rh(-) pregnant women, 56,6% were homozygous and 43,6%--heterozygous. The latter ones might have had either Rh(+) or Rh(-) children; in fact, in 52,2% of fetuses the D gene was not detected. Among fetuses of homozygous fathers (based on their phenotypes) 2 fetuses, to our surprise, occured to be Rh(-); however the subsequent genotyping showed that both fathers were heterozygous. The titres of anti-D in both groups of mothers with Rh(-) and Rh(+) fetuses were very similar. CONCLUSIONS: The examination of fetal D gene by noninvasive method should be performed in each alloimmunised Rh(-) mother if her partner is Rh(+). The prediction of RhD fetal status based on the fathers phenotype can be misleading, thus may result in unnecessary invasive method.
    [Abstract] [Full Text] [Related] [New Search]