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  • Title: [Spectrophotometric determination of chondroitin sulfate with victory blue B].
    Author: Chen YY, Jiang ZL, Li ZZ, Zhou SM, Sun SJ.
    Journal: Guang Pu Xue Yu Guang Pu Fen Xi; 2006 Jun; 26(6):1148-50. PubMed ID: 16961254.
    Abstract:
    In pH 4.0 acetic acid-sodium acetate buffer solution, cationic dye victory blue B shows an absorption peak at 614 nm, and the absorption peak decreases after it reacts with chondroitin sulfate to form association particles. The decrease in absorption value is linear with chondroitin sulfate concentration in the range of 0. 1-5 microg x mL(-1), and the correlation efficient is 0.9986. The method was applied to the determination of chondroitin sulfate in synthesis samples and real samples with rapidity, simplicity and good accuracy.
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