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  • Title: A modified process for preparing cationic polylactide-co-glycolide microparticles with adsorbed DNA.
    Author: Singh M, Fang JH, Kazzaz J, Ugozzoli M, Chesko J, Malyala P, Dhaliwal R, Wei R, Hora M, O'Hagan D.
    Journal: Int J Pharm; 2006 Dec 11; 327(1-2):1-5. PubMed ID: 16962267.
    Abstract:
    We have previously shown that cationic polylactide-co-glycolide (PLG) microparticles can be effectively used to adsorb DNA and generate potent immune responses in vivo. We now describe a modified and easier process containing a single lyophilization step to prepare these cationic PLG microparticles with adsorbed DNA. Cationic PLG microparticle formulations with adsorbed DNA were prepared using a modified solvent evaporation technique. Formulations with a fixed CTAB content and DNA load were prepared. The loading efficiency and 24h DNA release was evaluated for each formulation and compared to the earlier method of preparation. Select formulations were tested in vivo. The modified cationic PLG microparticle preparation method with a single lyophilization step, showed comparable physico-chemical behaviour to the two lyophilization steps process and induced comparable immune. The modified process with a single lyophilization step is a more practical process and can be utlized to prepare cationic PLG microparticles with adsorbed DNA on a large scale.
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