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  • Title: [Crude extraction and activity assay of CEL I].
    Author: Han SY, Yang ML, Gai JY, Yu DY.
    Journal: Yi Chuan; 2006 Sep; 28(9):1112-6. PubMed ID: 16963421.
    Abstract:
    CEL I, extracted from celery, is the first known eukaryotic nuclease that cleaves DNA with high specificity at sites of base-substitution mismatch and DNA distortion. It is a key enzyme for TILLING research. Here we reported a crude extraction method and activity assay of CEL I. Incision at mismatches of single nucleotide suggested that CEL I can effectively detect DNA at G-->A base substitution and the result can be obtained from an ABI377 Sequencer. Therefore, the extracted enzyme can be used in TILLING.
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