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  • Title: Preparation of single cell suspensions from hepatoma cells in culture.
    Author: Tokiwa T, Doi I, Sato J.
    Journal: Acta Med Okayama; 1975 Apr; 29(2):147-50. PubMed ID: 169672.
    Abstract:
    Fundamental examination was carried out on the liberation of single cells from hepatoma cells in culture. dRLa-74 cells derived from rat hepatoma, which hardly disperse as single cell suspensions with several proteolytic enzymes or EDTA alone, dispersed with a high yield of single cells by the combination of trypsin and EDTA. HUH-6 cells derived from human hepatoblastoma also showed similar results. The degree of cell dissociation by the combination was dependent on the incubation temperature or pH.
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