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  • Title: Effects of ethylene and 1-methylcyclopropene (1-MCP) on gene expression and activity profile of alpha-1,4-glucan-phosphorylase during banana ripening.
    Author: Mainardi JA, Purgatto E, Vieira A, Bastos WA, Cordenunsi BR, Oliveira do Nascimento JR, Lajolo FM.
    Journal: J Agric Food Chem; 2006 Sep 20; 54(19):7294-9. PubMed ID: 16968096.
    Abstract:
    Starch phosphorylases are enzymes that can use starch as substrate, and they are supposed to act in both in starch synthesis and degradation. This paper reports the effects of ethylene and 1-methylcyclopropene (1-MCP) on the degradation of starch and phosphorylase activity and gene expression. The results indicate that phosphorylase activity is induced during ripening and that it is associated with the onset of starch degradation. The regulation of banana phosphorylase activity is mainly dependent on gene expression, and the absence of ethylene perception by 1-MCP had a positive effect. However, this effect can be precluded by increased levels of ethylene, both autocatalytic and exogenous.
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