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  • Title: Probing rhodopsin-transducin interaction using Drosophila Rh1-bovine rhodopsin chimeras.
    Author: Natochin M, Barren B, Ahmad ST, O'Tousa JE, Artemyev NO.
    Journal: Vision Res; 2006 Dec; 46(27):4575-81. PubMed ID: 16979689.
    Abstract:
    Invertebrate and vertebrate rhodopsins share a low degree of homology and are coupled to G-proteins from different families. Here we explore the utility of fly-expressed chimeras between Drosophila rhodopsin Rh1 and bovine rhodopsin (Rho) to probe the interactions between the invertebrate and vertebrate visual pigments and their cognate G-proteins. Chimeric Rh1 pigments carrying individual substitutions of the cytoplasmic loops C2 and C3 and the C-terminus with the corresponding regions of Rho retained the ability to stimulate phototranduction in Drosophila, but failed to activate transducin. Surprisingly, chimeric Rho containing the Rh1 C-terminus was fully capable of transducin activation, indicating that the C-terminal domain of vertebrate rhodopsins is not essential for the functional coupling to transducin.
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