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  • Title: Keratinase of a streptomycete.
    Author: Mukhopadhyay RP, Chandra AL.
    Journal: Indian J Exp Biol; 1990 Jun; 28(6):575-7. PubMed ID: 1698173.
    Abstract:
    Keratinase produced from Streptomyces Sp.A11 decomposed human hair, chicken feather, wool, silk and pure keratin extracted from human epidermis. Purification of the enzyme by DEAE-cellulose column chromatography resulted in 7.5-fold increase in activity relative to the activity of the culture filtrate. The enzyme was inducible, extracellular, homogeneous with a molecular weight of 49,000. The enzyme activity was inhibited by reduced glutathione, phenylmethyl sulphonyl fluoride and 2-mercaptoethanol.
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