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  • Title: Single-particle evanescent light scattering simulations for total internal reflection microscopy.
    Author: Helden L, Eremina E, Riefler N, Hertlein C, Bechinger C, Eremin Y, Wriedt T.
    Journal: Appl Opt; 2006 Oct 01; 45(28):7299-308. PubMed ID: 16983418.
    Abstract:
    We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass-solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.
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