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  • Title: Evaluation of intracellular lipids by standardized staining with a Sudan black B fraction.
    Author: Subramaniam HN, Chaubal KA.
    Journal: J Biochem Biophys Methods; 1990 Jun; 21(1):9-16. PubMed ID: 1698849.
    Abstract:
    The selective staining of neutral lipids in Human Amnion cells in tissue culture was achieved using a particular fraction of the lipid dye, Sudan black B and a standardized staining procedure. The fraction, termed SBB-I, was isolated by thin-layer chromatography. The cytophotometric assessment of intracellular neutral lipids, stained with SBB-I, is described and applied to the study of changes in granulocytic neutral lipids in leukemia.
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