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  • Title: Vaccination of bovines with recombinant Boophilus Yolk pro-Cathepsin.
    Author: Leal AT, Seixas A, Pohl PC, Ferreira CA, Logullo C, Oliveira PL, Farias SE, Termignoni C, da Silva Vaz I, Masuda A.
    Journal: Vet Immunol Immunopathol; 2006 Dec 15; 114(3-4):341-5. PubMed ID: 16997384.
    Abstract:
    Boophilus Yolk pro-Cathepsin (BYC) is an aspartic proteinase found in Boophilus microplus eggs that is involved in the embryogenesis and has been tested as antigen to compose an anti-tick vaccine. The vaccine potential of a recombinant BYC expressed in Escherichia coli (rBYC) was investigated. rBYC was purified and used to immunize Hereford cattle. The sera of bovines immunized with rBYC recognized the native BYC with a titer ranging from 125 to 4000. Furthermore, immunized bovines challenged with 20,000 larvae presented an overall protection of 25.24%. The partial protection obtained against B. microplus infestation with the recombinant protein immunization was similar to the already described for native BYC immunization.
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