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Title: Assigning solid-state NMR spectra of aligned proteins using isotropic chemical shifts. Author: De Angelis AA, Howell SC, Opella SJ. Journal: J Magn Reson; 2006 Dec; 183(2):329-32. PubMed ID: 16997587. Abstract: A method for assigning solid-state NMR spectra of membrane proteins aligned in phospholipid bicelles that makes use of isotropic chemical shift frequencies and assignments is demonstrated. The resonance assignments are based on comparisons of 15N chemical shift differences in spectra obtained from samples with their bilayer normals aligned perpendicular and parallel to the direction of the applied magnetic field.[Abstract] [Full Text] [Related] [New Search]