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  • Title: Characterization of bovine seminal plasma by proteomics.
    Author: Kelly VC, Kuy S, Palmer DJ, Xu Z, Davis SR, Cooper GJ.
    Journal: Proteomics; 2006 Nov; 6(21):5826-33. PubMed ID: 17001600.
    Abstract:
    Previous investigations of bovine seminal plasma (BSP) have revealed the identities of the three major proteins, BSP-PDC109, BSP-A3 and BSP-30 kDa, which together constitute about half of the total protein, as well as about 30 of the minor proteins. Analyses of BSP by 2-DE have revealed about 250 protein spots, suggesting that much of the BSP proteome remains undescribed. In this study, BSP has been analyzed by 2-D LC-based and SDS-PAGE-based proteomic methods. Ninety-nine proteins were identified, including 49 minor proteins that have not previously been described in seminal plasma of any species.
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