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  • Title: [Expression of isoleucine zipper modified soluble CD40L in Pichia pastoris].
    Author: Du X, Tian L, Wang Y, Diao P, Wang G, Wei Y.
    Journal: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi; 2006 Aug; 23(4):844-7. PubMed ID: 17002122.
    Abstract:
    To obtain the expression of Isoleucine Zipper modified soluble CD40L (IZ-sCD40L) in Pichia pastoris, firstly, DNA fragment of IZ-sCD40L was obtained by PCR and over-lap PCR . Then the expression vector pPICZaA-IZ-sCD40L was constructed. Nucleotide sequencing analysis indicated that the DNA fragment of IZ-sCD40L was correctly inserted into the pPICZaA vector. Linearized pPICZ(alpha)A-IZ-sCD40L was introduced into Pichia pastoris GS115. Positive clone was selected by PCR and its phenotype was determined. The positive clone was introduced with methanol. The results of SDS-PAGE and Western blot showed that product was recombinant Isoleucine Zipper modified soluble CD40L fusion protein.
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