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  • Title: Osmium-zinc iodide reacts with interdigitating cells in the mouse lymph nodes and spleen.
    Author: Crivellato E, Mallardi F, Basa M, Zweyer M.
    Journal: Z Mikrosk Anat Forsch; 1990; 104(3):476-84. PubMed ID: 1700559.
    Abstract:
    It is known that epidermal Langerhans cells react with osmium-zinc iodide (ZIO) mixtures; therefore they can be visualized by this histochemical method. In the last few years it has been shown that Langerhans cells are closely related to the class of interdigitating cells (IDC) which are antigen presenting cells located in the T-dependent areas of lymph nodes and spleen. In this study the reactivity of murine IDC to ZIO has been assessed. Results demonstrate that ZIO procedure yields to a brilliant and selective staining of IDC. The reactivity pattern is quite similar to that previously observed in epidermal Langerhans cells. This finding gives further support to the concept that Langerhans cells and IDC are closely interrelated cell types.
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