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  • Title: High-performance liquid chromatographic determination of proteins by post-column fluorescence derivatization with thiamine reagent.
    Author: Yokoyama T, Kinoshita T.
    Journal: J Chromatogr; 1990 Sep 28; 518(1):141-8. PubMed ID: 1701770.
    Abstract:
    A post-column derivatization method for the high-performance liquid chromatography of peptides and proteins giving a fluorescence intensity proportional to the number of peptide bonds is described. Peptide bonds were chlorinated with hypochlorite and the N-chlorite formed was allowed to react with thiamine to give fluorescent thiochrome. This method was applied the determination of membrane-forming proteins of microorganisms.
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