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  • Title: A rapid HPLC/ESI-MS method for the quantitative determination of oridonin in rat plasma.
    Author: Xu W, Sun J, Zhang T, Tang J, Li H, Fang J, Chen D, He Z.
    Journal: Pharmazie; 2006 Sep; 61(9):757-9. PubMed ID: 17020150.
    Abstract:
    A rapid and accurate method using liquid chromatography with electrospray ionization mass spectrometric detection (HPLC/ESI-MS) was developed and validated for the determination of oridonin in rat plasma. The analytes were extracted with ethyl acetate-n-butyl alcohol (100:2, v/v) after spiking the samples with ethyl hydroxybenzoate (internal standard). The separation was carried out on a Diamon-sil C18 column with an isocratic mobile phase consisting of methanol-water (80:20, v/v) at a flow rate of 1.0 ml/min. The lower limit of quantification (LLOQ) of the method was 10 ng/ml and the linear range was 10-4000 ng/ml. The intra-day and inter-day accuracy and precision of the assay were less than 9%. This method has been applied successfully to a preliminary pharmacokinetic study involving the intravenous administration of oridonin to rats.
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