These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Cloning and functional expression of thermostable beta-glucosidase gene from Thermoascus aurantiacus.
    Author: Hong J, Tamaki H, Kumagai H.
    Journal: Appl Microbiol Biotechnol; 2007 Jan; 73(6):1331-9. PubMed ID: 17021873.
    Abstract:
    A thermostable beta-glucosidase (BGLI) was purified from Thermoascus aurantiacus IFO9748, and the gene (bgl1) encoding this enzyme was cloned and expressed in yeast Pichia pastoris. The deduced amino acid sequence encoded by bgl1 showed high similarity with the sequence of glycoside hydrolase family 3. The recombinant enzyme was purified and subjected to enzymatic characterization. Recombinant BGLI retained more than 70% of its initial activity after 1 h of incubation at 60 degrees C and was stable in the pH range 3-8. The optimal temperature for enzyme activity was about 70 degrees C and the optimal pH was about 5. P. pastoris expressing recombinant BGLI became able to utilize cellobiose as a carbon source.
    [Abstract] [Full Text] [Related] [New Search]