These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Expression and secretion of a single-chain sweet protein monellin in Bacillus subtilis by sacB promoter and signal peptide.
    Author: Chen Z, Heng C, Li Z, Liang X, Xinchen S.
    Journal: Appl Microbiol Biotechnol; 2007 Jan; 73(6):1377-81. PubMed ID: 17028871.
    Abstract:
    The sweet protein monellin gene was expressed in Bacillus subtilis under the control of the Bacillus subtilis sacB promoter and signal peptide sequence. A 294-bp DNA fragment, coding for sweet protein monellin, was ligated into the Escherichia coli/B. subtilis shuttle vector pHPC, producing pHPMS, which was subsequently transformed into B. subtilis QB1098, DB104, and DB403. The peptide efficiently directed the secretion of monellin from the recombinant B. subtilis cells. A maximum yield of monellin of 0.29 g protein l(-1) was obtained from the supernatant of B. subtilis DB403 harboring pHPMS. SDS-PAGE confirmed the purity of the recombinant product.
    [Abstract] [Full Text] [Related] [New Search]