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  • Title: Lactoperoxidase-catalyzed oxidation of thiocyanate by hydrogen peroxide: a reinvestigation of hypothiocyanite by nuclear magnetic resonance and optical spectroscopy.
    Author: Nagy P, Alguindigue SS, Ashby MT.
    Journal: Biochemistry; 2006 Oct 17; 45(41):12610-6. PubMed ID: 17029415.
    Abstract:
    In an effort to reconcile conflicting reports regarding the spectra of the human defense factor hypothiocyanite (OSCN(-)), we have synthesized OSCN(-) by three methods and characterized the product spectroscopically. Method I is lactoperoxidase-catalyzed oxidation of SCN(-) by H(2)O(2) at pH 7. Method II is hydrolysis of (SCN)(2) at pH 13. Method III is oxidation of SCN(-) by OX(-) (X = Cl and Br) at pH 13. All three methods produced essentially the same initial UV, (13)C NMR, and (15)N NMR spectra. The UV spectrum reveals a lambda(max) of 376 nm, which is a previously unreported distinguishing feature. The (13)C NMR spectrum (delta = 127.8 ppm at pH 13 vs dioxane at 66.6 ppm) is comparable to those that have been previously reported for OSCN(-) as prepared by methods I and II (although in some cases different assignments have been made). However, the (15)N NMR spectrum we measure (delta = -80.6 ppm at pH 13 vs NO(3)(-) at 0 ppm) contrasts with previous reports. We conclude that all three methods produce the same species, and the spectra are now self-consistent with the formulation OSCN(-).
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