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  • Title: Electrochemical studies on horseradish peroxidase covalently coupled with redox dyes.
    Author: Salomi BS, Mitra CK.
    Journal: Biosens Bioelectron; 2007 Mar 15; 22(8):1825-9. PubMed ID: 17029778.
    Abstract:
    The present study aims at investigating the use of redox dyes as non-diffusional electron mediators in hydrogen peroxide biosensors using horseradish peroxidase (HRP). We observe that the two redox dyes Safranine O and Neutral Red covalently bound to HRP, efficiently mediate electron transfer from the active site of the enzyme to the electrode surface. Dyes bound to the enzyme using a spacer arm diaminohexane further enhance the electron transfer. The enzyme electrodes show a linear response to the concentration of H2O2 up to 500 microM concentration and with a detection limit of around 50 microM. The dyes can be used as coupled mediators to develop a successful electro-optical biosensor.
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