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  • Title: Complementary segmental labeling of large RNAs: economic preparation and simplified NMR spectra for measurement of more RDCs.
    Author: Tzakos AG, Easton LE, Lukavsky PJ.
    Journal: J Am Chem Soc; 2006 Oct 18; 128(41):13344-5. PubMed ID: 17031932.
    Abstract:
    NMR structure determination of large RNAs is often restricted by limited RDC information caused by chemical shift degeneracy. We established a general, time- and cost-effective methodology for the preparation of 13C/15N complementary labeled RNAs from a single plasmid. Applying this method to the 25 kDa BC1-DTE RNA, we were able to resolve severe chemical shift degeneracy, thereby almost doubling the number of RDC restraints in comparison to the conventional 13C,15N uniform-labeled RNA.
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