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Title: Rose (Rosa hybrida L.). Author: Korban SS, Gasic K, Li X. Journal: Methods Mol Biol; 2006; 344():351-8. PubMed ID: 17033077. Abstract: Although rose transformation is successful, it remains difficult to transform myriad rose species as well as different rose genotypes. In this protocol, a detailed description of rose transformation is presented. This protocol relied on Agrobacterium-mediated transfer of embryogenic callus cultures. There are many critical steps that must be followed to achieve successful transformation; however, it is important to keep in mind that these apply to a selected number of genotypes, and as a different genotype is subjected to transformation, modifications of this protocol must be made to achieve successful transformation.[Abstract] [Full Text] [Related] [New Search]