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  • Title: [Quantification for biofilm formation of pseudomonas aeruginosa isolates and their genotypic analysis].
    Author: Cheng X, Yang WQ, Shi L, Yin XL, Xie Y, Zeng W, Li XH, Su JY, Kou YL, Jia WX.
    Journal: Sichuan Da Xue Xue Bao Yi Xue Ban; 2006 Sep; 37(5):666-9. PubMed ID: 17037723.
    Abstract:
    OBJECTIVE: To probe the correlation between the ability of biofilm formation of Pseudomonas aeruginosa isolates and their genotypes. METHODS: Forty-eight Pseudomonas aeruginosa isolates were tested for their biofilm formation with a modified microtiter test and were analyzed by enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The percent similarity between their genetic fingerprints and cluster analysis was performed and worked out using computer software. RESULTS: Forty-eight Pseudomonas aeruginosa isolates demonstrated different abilities of biofilm formation in vitro. And the 48 isolates with different abilities of biofilm formation showed different genotypes in fingerprints by ERIC-PCR. Among the 48 isolates of Pseudomonas aeruginosa tested, five clusters (A, B, C, D, E), were identified at the 17% genetic similarity level. The isolates with strong ability of biofilm formation were in D cluster at the 42% genetic similarity level. CONCLUSION: Most of the 48 PA isolates tested formed strong biofilm, and their genotypes were of correlation.
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