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  • Title: Ultra-sensitive colorimetric method to quantitate hundreds of polynucleotide molecules by gold nanoparticles with silver enhancement.
    Author: Li BS, Zhao LF, Zhang C, Hei XH, Li F, Li XB, Shen J, Li YY, Huang Q, Xu SQ.
    Journal: Anal Sci; 2006 Oct; 22(10):1367-70. PubMed ID: 17038778.
    Abstract:
    An ultra-sensitive colorimetric method to quantitate hundreds of polynucleotide molecules by gold nanoparticles with silver enhancement has been developed. The hybridization products from the target polynucleotides with biotin-labeled probes and gold nanoparticle-functioned oligonucleotides were immobilized to microplates via avidin-biotin system, and the absorbance signals of gold nanoparticles were amplified by silver enhance solution. This sandwich colorimetric assay can detect as few as 600 molecules for single-strand oligonucleotides and as few as 6000 molecules for double-strand polynucleotides in a 50 microL reaction system.
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