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Title: Identification of CT521 as a frequent target of Th1 cells in patients with urogenital Chlamydia trachomatis infection. Author: Olsen AW, Follmann F, Jensen K, Højrup P, Leah R, Sørensen H, Hoffmann S, Andersen P, Theisen M. Journal: J Infect Dis; 2006 Nov 01; 194(9):1258-66. PubMed ID: 17041852. Abstract: BACKGROUND: The human immune response to a Chlamydia trachomatis serovar D lysate was investigated in patients with urogenital C. trachomatis infection, to identify novel T cell targets. METHODS: A C. trachomatis lysate was fractionated on the basis of molecular mass, and each fraction was used to stimulate peripheral-blood mononuclear cells from patients with C. trachomatis infection. In frequently recognized fractions, proteins were identified by mass spectrometry, recombinantly expressed, and tested for T cell recognition. RESULTS: T cell recognition of the fractions was highly heterogeneous in patients with C. trachomatis infection (n=16). Four patients exhibited responses that were strongly targeted to antigens of 16-20-kDa molecular mass. Three proteins were identified in this fraction: CT043, CT511, and CT521. The T cell response to the individual recombinant proteins were investigated, and CT521 was found to induce the highest level of interferon (IFN)- gamma. The recognition of CT521 was investigated in a larger study population (n=41), and a positive IFN-gamma response was measured in 83% of the patients. Several T cell epitopes were identified in CT521; in particular, peptide 5 in the central part of the protein was frequently recognized by T cells (63%). CONCLUSION: We have identified a novel C. trachomatis antigen, CT521, that is frequently recognized in patients with urogenital C. trachomatis infection.[Abstract] [Full Text] [Related] [New Search]