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  • Title: Regeneration of adsorbed and covalently immobilized antibodies on solid phases for immunoassay.
    Author: Ilchmann D, Helbig D, Göhler H, Stopsack M, Thiele HJ, Hubl W.
    Journal: J Clin Chem Clin Biochem; 1990 Oct; 28(10):677-81. PubMed ID: 1704408.
    Abstract:
    A technique for the reproducible re-use of antibody-coated solid phases for immunoassays is described. The method based on the dissociation of the antigen-antibody complexes. Two different procedures, using glycine buffer (pH 2.3) or ethanolamine, were developed. More than ten immunoassay cycles can be realized with the same antibody-coated microtitre plates. These procedures were tested with competitive and sandwich immunoassays, monoclonal and polyclonal antibodies, and a commercial immunoassay kit.
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